Ehrlich ascites carcinoma (EAC)

Ehrlich ascites carcinoma (EAC) is one of the most common tumor models. EAC is known as undifferentiated carcinoma, originally hyperdiploid, with high transplantability, non-regression, rapid proliferation, short lifespan, 100% malignancy, and no tumor-specific transplantation antigen (TSTA). Typically, tumor virulence increases through repeated passages, while the proliferation rate of such tumors increases gradually. However, differentiation gradually disappears, while cells acquire free growth control mechanisms, acquire xenograftability, and eventually convert to the ascites form. EACs resemble the most chemosensitive human tumors in that they are undifferentiated and grow rapidly.

Signaling Pathways:

Several signaling pathways were reported to be dysregulated in EACs this includes Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL) and Fas Ligand.

Induction of Ehrlich ascites carcinoma

EAC cells are maintained by intraperitoneal (IP) passage of 1 X 106 cells in adult female Swiss albino mice. Seven days later, the ascetic fluid is collected from mice peritoneal cavity under sterile condition by needle aspiration. Mice are injected intraperitoneally (IP) with 1 X 106 EAC cells for tumor induction.