Bioassay-guided isolation of bioactive compounds from natural sources (plants, mushrooms as well as endophytic fungi, as a promising source for bio-pharmaceutics). 

Biotransformation.

Medicinal mushrooms.

Ethnopharmacology.

Functional foods

 

 

Lahloub M. F., Amer M. M., Zaki A., Ashour A., Amen Y., Samra R. M. Bioactive compounds from Malva rotundifolia L. World Journal of Pharmaceutical Sciences, 5 (8), 171-176 (2017).

Amen Y., Zhu Q., Tran H., Afifi M. S., Halim A. F., Ashour A., Fujimoto R., Goto T., Shimizu K. Rho-kinase inhibitors from Adlay seeds. Natural Product Research, (Accepted, In press).

Zhu Q., Amen Y., Ohnuki K., Shimizu K. Anti-influenza effects of Ganoderma lingzhi: An animal study. Journal of Functional Foods, 34, 224-228 (2017).

Tamrakar S., Nishida M., Amen Y., Tran H., Suhara H., Fukami K., Parajuli G., Shimizu K. Antibacterial activity of Nepalese wild mushrooms against Staphylococcus aureus and Propionibacterium acnes. Journal of Wood Science, 63 (4), 379-387 (2017).

Amen Y., Arung E., Afifi M. S., Halim A. F., Ashour A., Fujimoto R., Goto T., Shimizu K. Melanogenesis inhibitors from Coix lacryma-jobi seeds in B16-F10 melanoma cells. Natural Product Research, 31 (23), 2712-2718 (2017).

Kohno T., Tran H., Zhu Q., Amen Y., Sakamoto S., Tanaka H., Morimoto S., Shimizu K. Tubulin polymerization-stimulating activity of Ganoderma triterpenoids. Journal of Natural Medicines, 71 (2), 457-462 (2017).

Amen Y., Zhu Q., Tran H., Afifi M. S., Halim A. F., Ashour A., Shimizu K. Partial contribution of Rho-kinase inhibition to the bioactivity of Ganoderma lingzhi and its isolated compounds: insights on discovery of natural Rho-kinase inhibitors. Journal of Natural Medicines, 71 (2), 380-388 (2017).

Kishikawa A., Amen Y., Shimizu K. Anti-allergic triterpenes isolated from olive milled waste. Cytotechnology, 69 (2), 307-315 (2017). 

Amen Y., Zhu Q., Afifi M. S., Halim A. F., Ashour A., Shimizu K. New cytotoxic lanostanoid triterpenes from Ganoderma lingzhi. Phytochemistry Letters, 17, 64-70 (2016).

Mira A., Alkhiary W., Zhu Q., Nakagawa T., Tran H., Amen Y., Shimizu K. Improved Biological Activities of Isoepoxypteryxin by Biotransformation. Chemistry & Biodiversity, 13 (10), 1307-1315 (2016).

Amen Y., Zhu Q., Tran H., Afifi M. S., Halim A. F., Ashour A., Mira A., Shimizu K. Lucidumol C, a new cytotoxic lanostanoid triterpene from Ganoderma lingzhi against human cancer cells. Journal of Natural Medicines, 70 (3), 661-666 (2016).

Shimizu K., Amen Y. (equal contribution with the first author), Kaifuchi S. Methoxyflavones from New Lingzhi Medicinal Mushroom, Ganoderma lingzhi (Agaricomycetes). International Journal of Medicinal Mushrooms, 18 (8), 713-719 (2016).

Amen Y., Marzouk A. M., Zaghloul M. G., Afifi M. S. The genus Machaerium (Fabaceae): taxonomy, phytochemistry, traditional uses and biological activities. Natural Product Research, 29 (15), 1388-1405 (2015).

Amen Y., Marzouk A. M., Zaghloul M. G., Afifi M. S. A new acylated flavonoid tetraglycoside with anti-inflammatory activity from Tipuana tipu leaves. Natural Product Research, 29 (6), 511-517 (2015).
 

Amen Y. M., Marzouk A. M., Zaghloul M. G., Afifi M. S. Bioactive compounds from Tipuana tipu growing in Egypt. Journal of American Science, 9 (10), 334-339 (2013).